High-dimensional transcriptional analyses of UC Colon comparing RNALater to DMSO collection method

Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells. Multiple biopsies were taken from UC subjects to and placed into different collection media to compare the ability to extract total RNA and use total RNAseq to study the difference between inflamed and non-inflamed tissue