Additional file 2 of Long-term persistence of supernumerary B chromosomes in multiple species of Astyanax fish

Additional file 2. Dataset 1. Spreadsheet showing the procedure for CDS selection by applying consecutive filters: 1) All contigs showing mappings in gDNA libraries (40,679 contigs), 2a) Contigs with average number of copies, in the 0B libraries of A. scabripinnis, ranging between 0.5 and 4 (33,297 contigs), 2b) Contigs with average number of copies in the 0B libraries of A. paranae between 0.5 and 4 (32,844 contigs), 3a) Contigs showing genomic fold change due to B chromosome presence [gFC = log2(1B/0B)] higher than 0.585 in at least 1B individual of A. scabripinnis (1928 contigs), 3b) Contigs showing genomic fold change due to B chromosome presence [gFC = log2(1B/0B)] higher than 1 in the 1B individual of A. paranae (377 contigs), 4a) Contigs showing genomic fold change due to B chromosome presence [gFC = log2(1B/0B)] higher than 0.585 in all the three 1B individuals of A. scabripinnis (431 contigs), 5a) Contigs of A. scabripinnis annotated from Astyanax mexicanus genome excluding repetitive elements (227 contigs), 5b) Contigs of A. paranae annotated from Astyanax mexicanus genome excluding repetitive elements (257 contigs), 6) Combination of 5a and 5b data (450 contigs), 7) qPCR results associated with each contig. Note that the irl1b and lap4a contigs have gFC ≥ 0.585 in only two individuals of A. scabripinnis, while the urok gene meets this criterion in only one individual of this species, but the three genes have average gFC value about 0.65. Also note that the gFC of the urok gene in A. paranae is close to the threshold used.