mRNA profiling in Ustilago maydis under RUS (repopulation/regrowth under starvation)

mRNAs comparison between Ustilago maydis wild type grown in diluted YEPS (control) and in cell-free supernatants of Ustilago maydis wild type treated with H202 in two different concentrations (0.4% and 0.7%). Overall design: In order to identify genes differentailly expressed under RUS (repopulation/regrowth under starvation) Ustilago maydis wild type was grown for 16h in YEPS (1% (w/v) yeast extract, 2% (w/v) peptone, 2% (w/v) saccharose). The same amount of 2x107 cells of U.maydis wild type overnight culture was transfered to 10% YEPS (as control) as well as to cell-free supernatants of U.maydis wild type treated with H202 in two different concentrations (0.4% and 0.7%) (as two separate conditions). RNAseq analysis of all three type of samples (each analyzed in triplicates) focused on mRNA differentially expressed in two conditions vs. control.