Genome-wide identification of resistance genes and response mechanism analysis of key gene knockout strain to catechol in Saccharomyces cerevisiae

The engineering Saccharomyces cerevisiae applied for biodegradation and transformation of industrial toxic substances such as catechol has received widespread attention, but the low tolerance of S. cerevisiae to CA has limited its development. The exploration and modification of genes or pathways related to CA tolerance in S. cerevisiae is an effective way to further improve the utilization efficiency of CA. This study identified 36 genes associated with CA tolerance in S. cerevisiae through genome-wide identification and bioinformatics analysis, with the ERG6 knockout strain being the most sensitive to CA. The RNA-seq analysis of knockout strain under CA stress showed that some key pathways. This study provides a theoretical basis for improving the tolerance of strains to CA and reducing the damage of CA to the ecological environment and human health.