Cytokine profile of mature WT bone marrow-derived dendritic cells and fascin1 knockout bone marrow-derived dendritic cells

Cytokines secreted by WT and fascin1 KO BM-DCs maturated by LPS were determined by RayBio Quantibody mouse Th17 array1. Fascin1 KO BM-DCs show reduced secretion of several cytokines including IL-6. For analyses of cytokines in culture supernatants of BMDCs, WT and fascin1 KO BMDCs were cultured overnight after addition of LPS (100ng/ml). Culture supernatants were then used for profiling of inflammatory cytokines using a RayBio Quantibody mouse Th17 array1 according to the manufacturer’s instructions (RayBiotech). The Quantibody array allows us to quantitatively determine the concentrations of 18 cytokines/chemokines (IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, IL-17, IL17F, IL-21 IL-22, IL-23, IL-28, IFN, MIP-3, TGF1, and TNF).