Rok preferentially binds A+T-rich DNA in <i>E. coli</i>.

1The genetic locus in E. coli that was tested with PCR primers in quantitative real time PCR. The first three are in A+T-rich regions and the second three are in G+C-rich regions. Each primer set (Table S1) amplified a fragment between 101 bp–142 bp and the percent A+T of the amplified fragment is indicated. 2The overall % A+T for a region of the indicated size surrounding the amplified fragment is shown. % A+T was determined using SWAAP (Materials and Methods). 3The qPCR signal is indicated in arbitrary units based on a standard curve of total chromosomal DNA from the same strain. S.E. = standard error of the mean (n = 3). 4Data for ybcL and phnO are the same as those presented in Figure 4A for full length Rok-myc.