Elucidating the molecular pathology of DEPDC5 mutation induced mTOR-hyperactivation associated with epilepsy and autism spectrum disorder

To observe the effects of DEPDC5-KO on the transcriptome, 3?-mRNA analysis was performed Overall design: RNA was extracted from three biological replicates of D62-NTC, DEPDC5-2.1, and DEPDC5-2.2 with or without rapamycin progenitors and after 1 month of neuronal differentiation. Comparative gene expression profiling analysis of RNA-seq data for C3H10T1/2 cells and its KD derivatives (shKDM4D, shNFIB, and shMLL1).