Raman and EIS Dataset for the paper "Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment"

[EN] This is the experimental dataset used in the paper “Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment". The article includes the experimental procedure carried out for the deposition of graphene oxide and posterior immobilization of hyaluronic acid on CoCr surfaces. CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h. Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C for 24 h. Then, silanized CoCr surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 24 h. Carboxylic groups of graphene oxide in GO/CoCr samples were activated by immersing in distilled water (50 mL) with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC, 250 mg) and N-hydroxysuccinimide (NHS, 750 mg) during 24 h under mild orbital saking. After that, the GO/CoCr surfaces with activated carboxyl groups were immersed in distilled water (100 mL) with dissolved adipic dihydrazide (ADH, 1.04 g) and let to react for another 24 h under mild orbital shaking. Pre-activated HA-(EDC/NHS) solution was obtained by dissolving HA (100 mg), EDC (253 mg) and NHS (754 mg) in 100 mL phosphate buffer saline (PBS) and stirred for 24h to activate the carboxylic acids of HA. After that, ADH/GO/CoCr surfaces were immersed in pre-activated HA-(EDC/NHS) solution and let to react for 24h under mild orbital motion (samples named HA/GO/CoCr). This data set includes the results of the final surface by Raman Spectroscopy and Electrochemical Impedance Spectroscopy. Raman was obtained from CoCr/GO/HA surfaces in comparison with GO/CoCr surfaces and HA powder. Electrochemical impedance data of CoCr/GO/HA were obtained under immersion in 3 g/L Hyaluronic acid solution up to 23 days.