RPA-nfo and RPA-exo detection of <i>O</i>. <i>tsutsugamushi</i> DNA extracted from liver, spleen and lung of infected mice.

a Liver, spleen and lungs were collected at five time points before day 4 post infection. DNA from one mouse per time point was extracted as described in Materials and Methods and used for RPA reactions as described. Liver, spleen and lungs from five mice each at day 4, 7 and 11 post infection were collected. DNA was extracted as described to perform RPA reactions. b The copy number of O. tsutsugamushi 47 kDa gene was determined by qPCR (expressed as copy number per μL of DNA template) and presented as mean ± standard deviation of five mice. 5 μL of the DNA was used for RPA-exo or RPA-nfo detection. c ND: not detectable RPA-nfo and RPA-exo detection of O. tsutsugamushi DNA extracted from liver, spleen and lung of infected mice.