Arbuscular Mycorrhizal Fungal (AMF) Community During Crop Rotation, Lüneburg, Germany - OTU data

The dataset reports the contingency table of AMF sequence variants, their associated DNA sequence, accession number, and taxonomy. AMF come from soil or root samples from a two growing-season mesocosm experiment mimicking a crop rotation, with a first phase growing AM or non-AM and/or rhizobial or non rhizobial precrops followed with an AM crop. During the second phase, organic fertilization was applied (saw dust or wheat straw). The aim is to test the effect of precrop plant symbiotic functional group on following crop AM fungal diversity and abundance. Four crop plant species were used to have a combination of all symbiotic groups: spring canola (Brassica napus cv. Medicus, NPZ, non-rhizobial / non-AM), withe lupine (Lupinus albus cv. Energy, Feldsaaten Freudenberger, rhizobial / non-AM), spring barley (Hordeum vulgare cv. Barke, Saatzucht Breun, non rhizobial/AM) faba bean (Vicia faba cv. Tiffany, NPZ, rhizobial / AM). The succeeding crop was winter barley (Hordeum vulgare, cv. Antonella, Nordsaat Saatzucht, non-rhizobial / AM). The AMF community structure was analyzed by amplicon sequencing of the D1-D2 region of the fungal LSU (28S) rRNA gene using the primers LR2rev (5'-GAAAAGAACTTTGAAAA-3') and LR3 (5'-CCGTGTTTCAAGACG-3'). The taxonomy and sequence for each sequence variant is included. The sequences were generated using the primers LR2rev (5'-GAAAAGAACTTTGAAAA-3') and LR3 (5'-CCGTGTTTCAAGACG-3'). The results revealed that the mycorrhizal precrop symbiotic group (AM or not AM), but not the rhizobial one (N2-fixer or not N2-fixer), affected the richness and composition of AM fungi in the soil. The effect on composition, but not on richness, persisted in the following crop, demonstrating a legacy effect of precrop functional group. However, this effect was weaker than that of contemporary factors such as nutrient addition. AMF community composition correlated to decreasing crop yield after AM precrops. Phylogenetic analyses revealed that differences in AMF communities pertained at deep phylogenetic levels. These findings suggest negative plant-soil feedbacks within the AM symbiosis in agro-ecosystems typical of western Europe, driven by the consistent selection of deep clades of AM fungi. The raw fastq read files are available under BioProject accession number ENA PRJEB36419. The sequence variant sequences are available at ENA under accession number LR761341-LR761569. Related publications: (1) van Duijnen, R., Roy, J., Härdtle, W., & Temperton, V. M. (2018). Precrop Functional Group Identity Affects Yield of Winter Barley but Less so High Carbon Amendments in a Mesocosm Experiment. Frontiers in Plant Science, 9, 1–12. doi: 10.3389/fpls.2018.00912. (2) Roy, J., van Duijnen, R., Leifheit, E., Mbedi S., Temperton, V. M & Rillig, M.C. (accepted) Legacy effects of pre-crop plant functional group on fungal root symbionts of barley; Ecological Application.

Research domain: Soil Sciences

Research question: Do precrop have a legacy effect on the root fungal symbionts of the next crop?