Staphylococcus aureus strain:4185 Genome sequencing and assembly. Staphylococcus aureus strain:4185

Staphylococcus aureus 4185, a strain isolated from bovine mastitis in Brazil, exhibits potential biotechnological applications due to its antimicrobial activity against important pathogens, which is provided by the production of two antimicrobial peptides. One of them, named aureocyclicin 4185, is encoded on plasmid pRJ101 and seems to be a cyclic peptide, the first one described in staphylococci. Most of pRJ101 sequence was previously determined, except for ~100 bp, with a strong secondary structure. As pRJ101 does not carry genes involved in regulation of bacteriocin production, these genes might be encoded on the chromosome. Therefore, sequencing of the bacterial genome was expected: (i) to help to determine the complete nucleotide sequence of pRJ101, and to contribute to finding (ii) the regulatory genes involved in aureocyclicin 4185 production and/or (iii) a second bacteriocin gene cluster.