Raw data for 'Bacteria solve the problem of crowding by moving slowly'

These .zip files contain the raw data associated with the manuscript 'Bacteria solve the problem of crowding by moving slowly' (Meacock, O.J., Doostmohammadi, A., Yeomans, J.M., Foster, K.R., Durham, W.M., 2020). The contents consists of the following folders:-Cell morphology data: Data used to measure the length of WT and pilH cells at exponential-phase in liquid culture and in the monolayer. Used in Extended Data Fig. 6a.-Liquid culture competition CFUs: Spreadsheet containing the raw CFU counts associated with Extended Data Fig. 8b.-Low-density cell tracks: Tracks used to measure motility and shape of strains under low density subsurface conditions. Used in Extended Data Figs. 1c, 3b, 6a, 9d.-Monolayer cell and defect tracks: Defect and cell tracks from high-density WT and pilH subsurface monolayers. Used in Figs. 1g, 3e-f, Extended Data Figs. 3a and 4.-Raw confocal microscopy data: Raw confocal imaging files of surficial and subsurficial colonies. Used in Fig. 1d, Extended Data Fig. 2a.-Raw edge composition snapshots: Raw images used to generate Fig. 2g, Extended Data Fig. 10c-e.-Raw high-resolution rosette data: Raw and partially processed imaging data associated with the rosettes presented in Fig. 4d-f and Extended Data Fig. 7.-Subsurface colony composition and packing fraction data: Spreadsheets containing measurements of colony edge position, edge population composition and edge packing fraction, used to generate Fig. 2c-e, Extended Data Figs. 9b,c, 10a,b.-Subsurface colony edge coordinates: Spreadsheets containing measurements of colony edge position, used to generate Fig. 1f,g, Extended Data Fig. 1a,b.-Surficial colony composition measurements: Raw CFU counts and images used to generate Fig. 2a,b.For further details on the format of individual files, please refer to the readme.txt files in each folder.

这些.zip文件包含了与论文《细菌通过缓慢移动解决拥挤问题》（Meacock, O.J., Doostmohammadi, A., Yeomans, J.M., Foster, K.R., Durham, W.M., 2020）相关的原始数据。内容包含以下文件夹：
- 细胞形态数据：用于测量指数生长期在液体培养和单层培养中WT和pilH细胞长度的数据，用于扩展数据图6a。
- 液体培养竞争CFU：包含与扩展数据图8b相关的原始CFU计数的电子表格。
- 低密度细胞轨迹：用于测量低密度亚表面条件下菌株的游动性和形态的轨迹，用于扩展数据图1c、3b、6a、9d。
- 单层细胞和缺陷轨迹：来自高密度WT和pilH亚表面单层的缺陷和细胞轨迹，用于图1g、3e-f、扩展数据图3a和4。
- 原始共聚焦显微镜数据：表面和亚表面菌落的原共聚焦成像文件，用于图1d、扩展数据图2a。
- 原始边缘组成快照：用于生成图2g、扩展数据图10c-e的原始图像。
- 原始高分辨率花环数据：与图4d-f和扩展数据图7中呈现的花环相关的原始及部分处理后的成像数据。
- 亚表面菌落组成和填充分数数据：包含菌落边缘位置、边缘种群组成和边缘填充分数测量的电子表格，用于生成图2c-e、扩展数据图9b,c、10a,b。
- 亚表面菌落边缘坐标：包含菌落边缘位置测量的电子表格，用于生成图1f,g、扩展数据图1a,b。
- 表面菌落组成测量：用于生成图2a,b的原始CFU计数和图像。
有关单个文件格式的详细信息，请参阅每个文件夹中的readme.txt文件。