Whole-brain calcium imaging in freely-moving C. elegans during aversive copper boundary encounters

To identify candidate integrators of aversive stimuli, we performed whole-brain calcium imaging in freely-moving C. elegans. Animals were recorded while exploring arenas surrounded by copper. Calcium imaging of individual neurons in freely-moving C. elegans was conducted as previously described in Atanas et al., 2023. , , # Whole-brain calcium imaging in freely-moving C. elegans during aversive copper boundary encounters Dataset DOI: [10.5061/dryad.w9ghx3g4v](https://doi.org/10.5061/dryad.w9ghx3g4v) ## Description of the data and file structure ### Overview This dataset contains whole-brain neural activity recordings and behavioral measurements from individual *Caenorhabditis elegans* (*C. elegans*) worms freely moving in a circular arena surrounded by agar containing copper. Copper is aversive to *C. elegans*, and animals avoid the boundary when they encounter it. We imaged transgenic animals expressing pan-neuronal NLS-GCaMP7f and the NeuroPAL transgene, which provides fluorescent barcoding for neuron identification. This dataset contains data from 27 animals. Each animal is stored as one `.h5` file. Neuron identity assignments across all animals are stored in a single `.jld2` file. The .h5 files contain various measurements and analyses related to neural and behavioral data, including velocity an..., ,