Type II lung cells in young mice exposed to differing levels of oxygen
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
下载链接：
http://data.iscr.ac.cn/Article?id=99c301c216d018136e8f86584395d6ee
下载链接
链接失效反馈官方服务：
资源简介：
Alveolar epithelial type 2 (AT2) cells are an adult lung stem cell. Oxidative stress, mitogens, and cytokines affect their proliferation through poorly understood mechanisms. We previously showed in mice how neonatal hyperoxia between postnatal days (PND) 0-4 accelerates AT2 proliferation that normally takes place on PND7. Here, RNA-seq analysis of revealed neonatal hyperoxia stimulates expression of the mitochondrial-specific methylenetetrahydrofolate dehydrogenase (Mthfd) 2 and other genes involved in serine synthesis and one carbon-coupled folate metabolism. While MTHFD2 is important for embryonic development and proliferation of cancer cells, it is non-essential after birth. However, Mthf2 mRNA and other enzymes in serine and folate metabolism were highly expressed when AT2 cells proliferate on PND7. They were nearly undetectable in adult AT2 cells, but re-expressed when AT2 cells proliferated in response to fibroblast growth factor (FGF) 7 or during alveolar regeneration. Suppressing Mthfd2 or phosphoglycerate dehydrogenase (Phgdh) used to generate serine blocked FGF7-dependent proliferation of AT2 cells.
提供机构：
University of Rochester
创建时间：
2022-02-20
