rps16 analysis scripts and interval-reassembly results for Dioscorea

This dataset provides analysis scripts, supporting evidence, and interval-reassembly results for the rps16 investigation reported in the manuscript “Comparative plastomics of Dioscorea: genome conservation, sequence variation and an archived-read reassessment of rps16”. The deposit supports Figures 5–6, Supplementary Tables 12–19, and Supplementary Data 1. It includes 13 recovered matK–trnQ intervals, 52 native GetOrganelle files, archived-read assessment results, exon-detection evidence, coding matrices, fixed-topology ancestral-state reconstruction and sensitivity results, structural-analysis evidence, and scripts, inputs, and reference outputs for the D1/D2 calculations. The accompanying verification script checks file integrity, final plastome sequence lengths and coordinates, recovered-interval identities, and relevant scientific results. The supplied materials support rerunning the D1/D2 calculations. Complete reproduction of the analytical workflow from raw sequencing reads has not been demonstrated. The recovered sequences represent local intervals, not corrected whole plastomes, and do not establish rps16 gene function. The two newly assembled plastomes are available in GenBank under accession numbers QB228314 (Dioscorea nipponica, 153,918 bp) and QB228313 (D. quinquelobata, 153,945 bp). Their corresponding Illumina sequencing reads are available in the NCBI Sequence Read Archive under SRR41145379 and SRR41145380, respectively, associated with BioProject PRJNA1542792. Original analysis code is licensed under the MIT License, while original data and documentation are licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). Third-party materials retain their applicable licensing and attribution requirements.