Supplementary material for the paper ‘Pappalysins and stanniocalcins and their relationship with the peripheral IGF axis in newborns and during development’

This dataset is supplementary material for the paper: "Pappalysins and stanniocalcins and their relationship with the peripheral IGF axis in newborns and during development". It countains 4 figures and 2 tables
Background: Pappalysins (PAPP-A, PAPP-A2) modulate body growth by increasing IGF-I bioavailability through cleavage of IGFBPs and are inhibited by stanniocalcins (STC1, STC2). Normative data of these novel factors, as well as of free IGF-I and uncleaved fractions of IGFBPs are not well established. Objective: To determine serum concentrations of PAPP-A, PAPP-A2, STC1 and STC2, in relationship with other GH-IGF axis parameters during development. Patients and methods: Full-term newborns (150; gestational age: 39.30 ± 1.10 weeks), 40 preterm newborns (30.87 ± 3.35 weeks) and 1071 healthy individuals (1-30 years old) were included in the study and divided according to their Tanner stages (males and females): I:163 males, 154 females; II:100 males, 75 females; III:83 males, 96 females; IV: 77 males, 86 females; and V:109 males,128 females. Results: Serum concentrations of total and free IGF-I, IGF-II, total IGFBP-3 and ALS rose gradually during development peaking in Tanner stages III-IV. Concentrations of IGFBP-4 and IGFBP-5 were lower in prepubertal children. PAPP-A, PAPP-A2, STC1, STC2, IGFBP-2, total IGFBP-4 and total IGFBP-5 were elevated at birth and declined throughout childhood. In postnatal life, PAPP-A2 concentrations decreased progressively in concomitance with the free/total IGF-I ratio; however, stanniocalcin concentrations remained stable. PAPP-A2 concentrations positively correlated with the free/total IGF-I ratio (r=+0.28 p<0.001) and negatively with the intact/total IGFBP-3 ratio (r=-0.23, p<0.001). PAPP-A concentrations inversely correlated with intact/total IGFBP-4 ratio (r=-0.21, p<0.001), with PAPP-A concentrations being lower in females at all ages. Conclusion: This study provides reference values of pappalysins and stanniocalcins, which modulate IGF-I activity by changing the concentrations of uncleaved IGFBPs.

本数据集为论文《Pappalysins 和 Stanniocalcins 及其与新生儿及发育过程中周围 IGF 轴的关系》的补充材料。该数据集包含 4 幅图表和 2 张表格。

背景：Pappalysins（PAPP-A、PAPP-A2）通过切割 IGFBPs 增加IGF-I的生物利用度，从而调节身体生长，并被 Stanniocalcins（STC1、STC2）所抑制。这些新型因子的规范数据，以及游离IGF-I 和未切割的 IGFBPs 的分数尚未得到充分确立。

研究目标：确定 PAPP-A、PAPP-A2、STC1 和 STC2 的血清浓度，在发育过程中与其他 GH-IGF 轴参数的关系。

研究对象与方法：本研究纳入了 150 名足月新生儿（妊娠期：39.30 ± 1.10 周）、40 名早产新生儿（妊娠期：30.87 ± 3.35 周）和 1071 名健康个体（1-30 岁），并根据他们的 Tanner 阶段（男性和女性）进行分组：I 阶段：163 名男性，154 名女性；II 阶段：100 名男性，75 名女性；III 阶段：83 名男性，96 名女性；IV 阶段：77 名男性，86 名女性；V 阶段：109 名男性，128 名女性。

结果：总 IGF-I、游离 IGF-I、IGF-II、总 IGFBP-3 和 ALS 的血清浓度在发育过程中逐渐上升，在 Tanner 阶段 III-IV 达到峰值。IGFBP-4 和 IGFBP-5 的浓度在青春前期儿童中较低。PAPP-A、PAPP-A2、STC1、STC2、IGFBP-2、总 IGFBP-4 和总 IGFBP-5 在出生时升高，并在儿童期持续下降。在出生后生活中，PAPP-A2 的浓度随着游离/总 IGF-I 比例的降低而逐渐下降；然而，Stanniocalcin 的浓度保持稳定。PAPP-A2 的浓度与游离/总 IGF-I 比例呈正相关（r=+0.28，p<0.001），与完整/总 IGFBP-3 比例呈负相关（r=-0.23，p<0.001）。PAPP-A 的浓度与完整/总 IGFBP-4 比例呈负相关（r=-0.21，p<0.001），且在所有年龄段女性的 PAPP-A 浓度均较低。

结论：本研究提供了调节 IGF-I 活性的 Pappalysins 和 Stanniocalcins 的参考值，这些因子通过改变未切割 IGFBPs 的浓度来实现这一调节作用。