Dissecting cell composition and cell-cell interaction network of human disease heart tissue by single-cell sequencing

We studied the cell compositon of two types of human heart disease (coronary atherosclerotic heart disease and dilated cardiomyopathy) by single-cell sequencing. Distinct subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We generated a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with age. Overall design: Extract cells from left ateria and ventricle of human heart tissue and conduct single-cell sequencing