Pseudomonas entomophila strain:23S Genome sequencing

Biopesticides, which consist of antagonistic microorganisms or bioactive compounds they produce, offer attractive alternatives to synthetic agrochemicals as a means of reducing plant diseases in crop production systems. In our previous study, a strain of Pseudomonas sp., 23S, was found to have a strong antagonistic activity toward Clavibacter michiganensis subsp. michiganensis (Cmm), a bacterial pathogen of tomato causing canker disease, which results in serious damage to the tomato production industry. This study focused on purification and characterization of the anti-Cmm compound(s) produced by this Pseudomonas sp. 23S. Whole genome sequencing was conducted to confirm identification at species level as Pseudomonas entomophila 23S and to examine the bacterium's potential for disease control from predicted gene functions; antiSMASH analysis suggests that the genome contains four clusters of antimicrobial peptides/metabolites, some of which are yet to be identified, implicating a potential for a few newer antimicrobial compounds that can be isolated and characterized.