Data from: Solenopsis invicta Viruses and Kneallhazia solenopsae in Tennessee Imported Fire Ant (Hymenoptera: Formicidae) Populations

The dataset contains the geographic occurrence of Kneallhazia solenopsae (Knell, Allen & Hazard) (Microsporidia: Burenellidae) (formerly Thelohania solenopsae Knell Allen & Hazard) and Solenopsis invicta virues (SINV-1, SINV-2, and SINV-3) pathogens, as well as Pseudacteon spp. (phorid decapitating fly) presence in imported fire ant (Hymenoptera: Formicidae) colonies sampled throughout the entire Tennessee Federal Imported Fire Ant Quarantine (FIFAQ) area in 2015 to 2016. Pathogen and phorid presence were determined by PCR. Colonies were sampled from July to October in 2015 and 2016 (warm period) (n=440 colonies) and January to April in 2016 (winter period) (n=227 colonies) using a sampling grid of 12.1 by 12.1 kilometers placed over all locations in Tennessee within FIFAQ. During the cool period, every other grid was sampled resulting in a lower total sample number. The project goal was to determine geography and seasonality of pathogens and phorids in Tennessee fire ant populations.
The dataset also contains venom alkaloid (IALK) and cuticular hydrocarbon (IHC) values for each fire ant colony sampled as determined by a Shimadzu QP-2010 Gas Chromatograph - Mass Spectrometer (GC-MS), which were used to determine if samples are red imported fire ant (Solenopsis invicta Buren) (RIFA), black imported fire ant (Solenopsis richteri Forel) (BIFA), or the hybrid imported fire ant (HIFA) of the two parental species. The IALK and IHC values were used to calculate an average (IAVG) and adjusted hybridity index value for each colony sampled with the adjusted value being used to determine BIFA, HIFA, or RIFA status.
The dataset also contains a random sub-sample of ant colonies (n=99) across hybridity groupings (described in Pandey et al. [2019]: https://doi.org/10.1093/ee/nvz023 ) analyzed for the colony social form by analysis of the Gp-9 locus for B (monogyne [single queen]) and b (polygyne [multi-queen) alleles.
Other dataset items include the sampling date, the colony grid number, the colony county location and general state region (east, middle, west), global positioning system coordinates for each colony sample location, and colony elevation (meters and feet). A general sample site description is also provided for each sample site location.
Gaps in the dataset include sample grids where imported fire ant colonies could not be located (indicated by "No_" in the “Sampling_Successful” column). For samples where no fire ant colony could be located, the GPS coordinates and sample elevation are still provided using the grid point location, as well as the date of the attempted colony sampling. The dataset also does not contain any data for areas in-between grid points that were not sampled. In some cases where fire ant colonies could not be located near the grid point location, colonies were collected where they could be found as close to the grid center as possible.

Funding provided by:
USDA Animal Plant Health Inspection Service Plant Protection and Quarantine Agreement No. 15-8130-0523-CA entitled "Distribution of Solenopsis invicta virus (SINV) and Kneallhazia solenopsae in Tennessee and potential relocation of virus into new areas".
USDA Floriculture and Nursery Research Initiative (U.S. National Arboretum CRIS #8020-21000-086-000D). Agreement No. 58-8020-8-009 entitled "Methods for Quarantine Certification and Biological Control of Imported Fire Ant in Nursery Production Systems".
USDA-ARS Southern Horticultural Research Unit (NACA entitled "Enhancing Sustainability and Efficiency of Nursery Plant Production in the Southeast U.S.". Agreement number 58-6062-3-003. Parent CRIS #6062-21430-004-00D entitled "Management of Diseases, Pests, and Pollinators in Horticultural Crops".
USDA National Institute of Food and Agriculture Evans-Allen (Accession numbers 1004787 and 1014556)

本数据集涵盖了2015至2016年期间，在田纳西联邦进口火蚁检疫区（FIFAQ）内采集的Kneallhazia solenopsae（Knell, Allen & Hazard）[Microsporidia: Burenellidae]（此前名为Thelohania solenopsae Knell Allen & Hazard）及Solenopsis invicta virues（SINV-1、SINV-2和SINV-3）病原体在地理分布上的出现情况，以及伪阿蜂属（phorid decapitating fly）在进口火蚁（Hymenoptera: Formicidae）群体中的存在情况。这些数据由2015年7月至10月（温暖季节）（n=440个群体）和2016年1月至4月（冬季）（n=227个群体）在田纳西州FIFAQ区域内的12.1公里×12.1公里的采样网格所采集。在凉爽季节，每隔一个网格进行采样，从而降低了总样本量。项目的目标是确定田纳西火蚁群体中病原体和伪阿蜂的地理分布和季节性特点。
数据集还包含了每个被采样火蚁群体的毒液生物碱（IALK）和表皮烃（IHC）值，这些值通过Shimadzu QP-2010气相色谱-质谱联用（GC-MS）测定，并用于判断样本是否为红色进口火蚁（Solenopsis invicta Buren）（RIFA）、黑色进口火蚁（Solenopsis richteri Forel）（BIFA）或两个亲本种类的杂交进口火蚁（HIFA）。IALK和IHC值被用于计算每个采样群体的平均（IAVG）和调整后的杂交指数值，以确定BIFA、HIFA或RIFA的状态。
此外，数据集还包含了来自不同杂交分组（详见Pandey等[2019]：https://doi.org/10.1093/ee/nvz023）的随机亚样本（n=99）的蚂蚁群体，通过分析Gp-9基因座上的B（单后）和b（多后）等位基因来研究群体的社会形态。
数据集的其他项目还包括采样日期、群体网格编号、群体所在县和一般州域（东部、中部、西部）、每个群体样本位置的全球定位系统坐标以及群体海拔（米和英尺）。同时，还为每个采样地点提供了简要的现场描述。
数据集中存在的一些空白包括无法定位进口火蚁群体的采样网格（在“Sampling_Successful”列中用“No_”表示）。对于无法找到火蚁群体的样本，仍提供了GPS坐标和样本海拔，以及尝试采样群体的日期。此外，数据集未包含未采样的网格点之间的区域数据。在某些情况下，如果火蚁群体无法在网格点附近找到，则会在尽可能接近网格中心的位置收集群体。
资金支持来源包括：美国农业部动植物卫生检验局植物保护与检疫协议号15-8130-0523-CA，项目名称为“田纳西州Solenopsis invicta病毒（SINV）和Kneallhazia solenopsae的分布及其可能的新区域病毒转移”；美国农业部花卉和苗圃研究倡议（美国国家植物园CRIS #8020-21000-086-000D），协议号58-8020-8-009，项目名称为“苗圃生产系统中进口火蚁的检疫认证和生物控制方法”；美国农业部-ARS南部园艺研究单位（NACA项目名称为“提高美国东南部地区苗圃植物生产可持续性和效率”，协议号58-6062-3-003，CRIS #6062-21430-004-00D，项目名称为“园艺作物疾病、害虫和传粉者的管理”；美国农业部国家粮食与农业研究所Evans-Allen（登录号1004787和1014556）。