HT1080WT cells embedded in 3D collagen type I matrices - manual annotations for cell instance segmentation and tracking

Human fibrosarcoma HT1080WT (ATCC) cells at low cell densities embedded in 3D collagen type I matrices [1]. The time-lapse videos were recorded every 2 minutes for 16.7 hours and covered a field of view of 1002 pixels × 1004 pixels with a pixel size of 0.802 μm/pixel The videos were pre-processed to correct frame-to-frame drift artifacts, resulting in a final size of 983 pixels × 985 pixels pixels.

Hasini Jayatilaka, Anjil Giri, Michelle Karl, Ivie Aifuwa, Nicholaus J Trenton, Jude M Phillip, Shyam Khatau, and Denis Wirtz. EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration. FASEB J., 32(3):1207–1221, 2018. ISSN 0892-6638. doi: 10.1096/fj.201700444RR.

Further information about how to use this data is given in https://github.com/esgomezm/microscopy-dl-suite-tf

This dataset is provided together with the following preprint and if you use it, we would like to kindly ask you to cite it properly:

Estibaliz Gómez-de-Mariscal, Hasini Jayatilaka, Özgün Çiçek, Thomas Brox, Denis Wirtz, Arrate Muñoz-Barrutia, *Search for temporal cell segmentation robustness in phase-contrast microscopy videos*, arXiv 2021 (arXiv:2112.08817)