An image-based screen for secreted proteins involved in breast cancer G0 cell cycle arrest

To identify novel secreted proteins that can modulate breast cancer G0 arrest, we performed a secretome-wide, image-based screen for proteins which alter the balance between proliferation and G0 arrest. From a secretome library of 1282 purified proteins, we identified 29 candidates that promote G0 arrest in non-transformed (hTERT-HMEC) and transformed (MCF7) breast epithelial cells.