Transcriptome of zeta Trypsin RNAi (PRJCA042621)

In ?Trypsin knockdown testes, spermatid individualization complexes with F-actin cones were either entirely absent or disrupted, leading to an absence of mature sperms in the seminal vesicle, and resulting in male sterility. The most significant effects included reduced tubulin polyglycylation and disrupted mitochondrial function. Transcriptome analysis identified 1878 differentially expressed genes, with 814 genes upregulated and 1064 genes downregulated. These findings suggest that ?Trypsin is essential for spermatid maturation by influencing mitochondrial morphogenesis.