RNA-seq of coding RNA of wheat heads from 3, 6, 12, 24, 36, and 48 hours after inoculation of fungal pathogen Fusarium graminearum or mock inoculation. RNA-seq of coding RNA of wheat heads from 3, 6, 12, 24, 36, and 48 hours after inoculation of fungal pathogen Fusarium graminearum or mock inoculation

Near isogenic wheat lines, differing in the presence of both or none of the FHB-resistance QTL Fhb1 and Qfhs.ifa-5A, have been sequenced using Illumina HiSeq2000 under disease pressure (3, 6, 12, 24, 36, 48 hai) as well as with mock-inoculation, to discern transcriptional differences induced by Fusarium graminearum. The NILs are BC5F2 lines generated from the Mexican Spring wheat line CM-82036, the resistance QTL donor line, as recurrent background and the susceptible German Spring wheat line Remus as the donor of the susceptible QTL alleles.