ACP mutants.

ACP mutants number 3 to 149 were obtained by random mutagenesis. Mutants ACP745 and ACP749 were obtained by site-directed mutagenesis. Mutant ACP(S36T) was described previously [15]. Functionality corresponds to the result of the complementation test by transduction of the ΔacpP::kanaR deletion in strain MG1655 containing the pKO3-acpP mutant construction (see text). + indicates growth on LB plate equivalent to the growth of the wild type; -indicates that no clone was obtained; +/− indicates that colonies were obtained, but growth was severely delayed; nd (not determined) indicates that the corresponding ACP mutants were not tested for functionality. The six mutants further characterized in the study are indicated in bold letters.