A pathogenic CD4 T cell phenotype in experimental uveitis shares common features with other immune mediated inflammatory diseases [EAU]

Experimental autoimmune uveoretinitis, a mouse model of ocular autoimmunity, was induced by the transfer of allelically marked T cells. Thirteen days after transfer, CD4 cells were sorted from spleens and individual eyes and processed using the SMART-Seq v4 Ultra Low Input RNA kit. Samples were classified as: transferred cells from the eye, transferred cells from the spleen, endogenous cells from the eye, endogenous cells from the spleen. Analysis of differential gene expression revealed an extended pathogenic phenotype that shared common features with other models of autoimmunity. Overall design: Data from 24 samples obtained from two experiments (ASS03 & ASS04) and classified as transferred CD4 cells from single eyes (e4T), transferred CD4 cells from the spleen (s4T), endogenous cells from the eye (e4E) and endogenous cells from the spleen (s4E). Cells numbers were in the range 18-100 cells.