Identification of H3K27ac marks influenced by FOXA2 and/or mutant KRAS in BEAS-2B transformed human bronchial epithelial cells

In order to identify H3K27ac enhancer marks influenced by FOXA2 and/or KRAS.G12V, BEAS-2B cells were infected with lentivirus carrying rat FOXA2 and/or retrovirus carrying human KRAS.G12V. FOXA2 and/or KRAS.G12V-expressing BEAS-2B cells were used for chromatin immunoprecipitation-sequencing (ChIP-seq) to detect genome-wide H3K27ac positive regions. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modification H3K27ac in BEAS-2B cells stably-expressing ectopic FOXA2 and/or KRAS.G12V.