m6A-RIP-seq of mRNA in yw, Mettl3, Mettl14 and Hakai flies

In order to obtain a stringent m6A methylome in Drosophila adults, we performed m6A-RIP-seq in yw control (male and female), Mettl3 (male), Mettl14 (male) and Hakai (male) mutant flies. We find that the effective m6A modification, which depends on the writer complex, is mostly distributed in 5’ UTR and near start codon in Drosophila, in contrast to the mammalian system. We define a set of high-confident m6A methylation sites shared by Mettl3, Mettl14 and Hakai, indicating that Hakai is a core component of the m6A writer complex. We also find differential methylation pattern in certain loci between male and female flies. Flies of 4 genotypes were analyzed with two replicates. For yw, experiments were performed on both male and female flies. For Mettl3, Mettl14 and Hakai, only males were used. Each RNA preparation was divided into input and IP samples, so total samples were 20.