Ultrafast and Accurate DNA Storage and Reading Integrated System Via Microfluidic Magnetic Beads Polymerase Chain Reaction

DNA storage is expected to tackle
the dilemma faced by
electronic
information technology for the effective storage and management of
massive amounts of data in the era of big data. Efficient and reliable
data retrieval is crucial for DNA storage. However, it is still challenging
to actualize DNA storage with fast and accurate readout capabilities,
which play a key role in the practicality and reliability of DNA storage.
In this study, an integrated system was constructed using homemade
microfluidic PCR and DNA magnetic beads for fast and accurate DNA
storage and reading with reproducibility. The homemade microfluidic
PCR and DNA magnetic beads constructed for the random access of DNA
storage have the advantages of short time and low bias named MMBP.
The homemade DNA magnetic beads are low cost, stable, and reproducible.
The integrated DNA storage and reading system integrated by MMBP can
read information not only more accurately and quickly but also at
a lower sequencing depth than traditional PCR. Overall, the MMBP-based
DNA information storage system (MMBP-DIS) has the advantages of reducing
the cost, decreasing the random access time to 10 min, and improving
the reading accuracy and sensitivity. In the future, it can be integrated
with DNA electrochemical synthesis to develop a fast and accurate
portable microfluidic device for DNA synthesis-preservation-reading
integration.