Genome-wide acetylation of histone 3 at lysine residuce (H3K27ac) deposition in human iPS cell-derived cardiomyocytes (hiPSC-CMs)

To determine the role of estrogen-related receptor gamma (ERRg) in cardiac myocyte enhancers, we performed ChIP-seq studies with hiPSC-CMs using antibody directed against acetylated histone 3 at lysine residue 27 (H3K27ac), a known active enhancer mark. We found that knocking out ERRg downregulated H3K27ac depositions on several adult cardiac contractile protein and mitochondrial oxidative metabolic genes. These results establish significant roles of ERR to activate cardiac enhancer and promoter activities. Overall design: With ChIP-seq, examination of H3K27ac deposition on the human genome in wild type hiPSC-CMs and ERRg KO hiPSC-CMs.