Neddylation Signaling Inactivation by Tetracaine Hydrochloride Suppresses Cell Proliferation and Vemurafenib-resistance of Melanoma

This study aimed to investigate the transcriptomic changes in A375 cells upon treatment with 400µM tetracaine hydrochloride (TTC) for 24 hours. RNA-seq data were generated from TTC-treated and untreated control A375 cells to identify differentially expressed genes and pathways involved in the cellular response to TTC exposure. The findings may provide insights into the molecular mechanisms underlying the effects of TTC on A375 cells. Overall design: The experiment involved two conditions: 1) A375 cells treated with 400µM TTC for 24 hours, and 2) A375 cells treated with an equivalent dose of PBS as a control. Three biological replicates were prepared for each condition. Total RNA was extracted, and RNA-seq libraries were constructed and sequenced using Illumina technology.