ATAC-Seq of liver in cancer cachexia

To study the response in the liver to cachectic and weight-stable cancer, we performed ATAC-sequencing of hepatocyte nuclei that were isolated using the INTACT methodology of weight stable NC26, pre-cachectic C26, cachectic C26, weight-stable MC38 and cachectic 8025 tumor bearing mice as well as their respective PBS controls. Overall design: We implanted cachexia-inducing tumor cells (C26, 8025) or tumor cells that give rise to weight-stable cancer (NC26, MC38) into syngenic Hep-INTACT mice. Livers were collected once a body weight loss of >10% was reached for C26 and 8025 tumor bearing animals at zeitgeber zeit (ZT) 9.