Resource limitation rewires chromosome instability and ploidy evolution across in vitro and in vivo cancer models

This dataset contains the processed single-cell RNA-sequencing data used to generate Figure 7. It includes 18 sample-level velocyto .loom files generated from 10x Genomics outputs, containing spliced and unspliced RNA counts for RNA-velocity and scVelo analyses, together with the final integrated Seurat .rds object used for cell annotation, clustering, UMAP/PCA visualization, and downstream trajectory and pseudotime analyses. The data were derived from orthotopic breast cancer models under vehicle and gemcitabine treatment conditions and include relevant sample, treatment dose, cluster, and ploidy metadata. README files, data-generation parameters, software and runtime provenance, and SHA-256 checksum manifests are provided to support data integrity and reproducibility.