qRT-PCR

To assess whether the differentially expressed miRNAs were consistent with the gene chip results, we validated rno-miR-3068-3p expression via qRT‒PCR. The results revealed that the rno-miR-3068-3p expression level was higher in the I/R group than in the sham group and that CIP led to the downregulation of rno-miR-3068-3p expression in rat hippocampal tissues (P < 0.05) . The trend of the rno-miR-3068-3p change was consistent with the gene chip results, confirming the reliability of the gene chip study results.