The inferred interaction status of <i>S</i>. <i>cerevisiae</i> paralogous pairs in PPI data detected exclusively by methods other than two-hybrid and pulldowns.
aThe presence of FRET was indicated by a decrease (>10%) in the ratio of emission at λ476/emission at λ 526 of corrected CFP (CFP in the CFP and YFP mixture after subtraction of the contribution due t
We have defined inactive α and ω fragments of β-lactamase that can complement to form a functional enzyme in both bacteria and mammalian cells, serving as a readout for the interaction of proteins fus
PLA was performed in HEK 293 cells transfected with ASRGL1 and TDP-43 plasmids and incubated with antibodies: no primary, only TDP-43, only ASRGL1, TDP-43 + IgG and ASRGL1 + TDP-43.
We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its str