Knockdown analysis of <i>CPIJ005623</i> in <i>C. pipiens</i> males.

A. Diagram representing experimental design of knockdown (KD) experiments, d-day post pupal eclosion, BF- Blood feed, T-time point. B. KD assessment of iCPIJ005623 in Pel testis. Reduction of CPIJ005623 mRNA levels was observed in testis when CI developmental progression was seen. C–F Embryo developmental progression after iCPIJ005623 males in an incompatible Italy female cross. Timepoints under dsRNA early effect (C-T1; D-T2) show an increased proportion of Stage II&III embryos compared to control KDs. No effect was seen at later time points (E-T3; F-T4). G. KD assessment of iCPIJ005623 in Italy testes. H. Double KD analysis of CPIJ005623 in Pel females crossed to Italy males. Increased developmental progression is detected in female, male and double iCPIJ005623.