BTA3 control tiller angle formation and shoot gravitropism in rice

To explore the roles of BTA3 in regulating tiller angle, we performed RNA high-throughput sequencing analysis. The response of wild type (WT) and bta3-1 mutant to gravity stimulation was investigated in the study. Overall design: Two group of seedlings were used for RNA-seq with two biological replicates. One group is the vertical-grown 7-day-old WT and bta3-1 seedlings serving as no gravity stimulation control, named YJWC1, YJWC2; YJBC1, YJBC2. The other group is the 7-day-old WT and bta3-1 seedlings, which were vertical-grown for 4 days and transferred to horizontal-grown status for another 3 days serving as gravity stimulation treatment, named YJWT1, YJWT2; YJBT1, YJBT2.