Dual oxidase is essential for molting, hatching and feeding in the brown planthopper

Dual oxidase (Duox) is well-known for its role in immunity and tyrosine crosslinking activity across various biological processes from mammals to holometabolous insects. Nevertheless, its function in hemimetabolous insects remains poorly understood. In this study, we explored the physiological roles of the Duox gene in a hemimetabolous insect, the brown planthopper. We conducted series of experiments, this dataset is the experimental data derived from our manuscript, including qRT-PCR data, survival rate data, hydrogen peroxide content data, body weight and length data, feeding behavior related data, and reproduction related data., For qRT-PCR data, relative transcript levels were calculated using the 2−ΔΔCt method. All data were expressed as mean ± SD, based on at least three replicates. Statistical analyses were performed using GraphPad Prism 8.0 (GraphPad Software, CA, USA). Comparisons between two treatments were conducted using Student’s t test or Welch’s t test. Multiple comparisons were analyzed using one-way ANOVA with Games-Howell post hoc tests for non-homogeneous variances. Statistical significance was set at p < 0.05 (*) and p < 0.01 (**)., # Dual oxidase is essential for molting, hatching and feeding in the brown planthopper

Dataset DOI: [10.5061/dryad.q2bvq83ww](10.5061/dryad.q2bvq83ww)

## Description of the data and file structure

We conducted series of experiments，this dataset is the experimental data derived from our manuscript, including qRT-PCR data (Temporal/Tissue-specific/Ovarian/RNAi efficiency), survival rate data, hydrogen peroxide content data, body weight and length data, feeding behavior related data, Vc treatment and hydrogen peroxide rescue data, and reproduction related data.
### Files and variables

#### File: 5th\_instar\_H2O2\_measurement.csv

**Description:** Hydrogen peroxide level was measured 84 h after dsRNA-injection or at 0/48/84 h 5th-instar nymphs.

##### Variables

* H2O2 level (μmol/g) 

#### File: Body\_length\_and\_weight.csv

**Description:** Body length and weight of females were measured. 

##### Variables

* length(mm)
* weight(mg)

#### File: EPG\_and\_salivary\_sheath.csv

**De...,