Determination of different gene expressions for AngII induced wild-type and smcKlf5-/- mouse abdominal aortic aneurysm

To identify genes that are regulated by KLF5 during vascular smooth muscle cell mitochondrial mophology, redox and ATP, we performed RNA expression profiling using microarray analyses of AAA and normal abdominal aortas from WT (control) and smcKlf5−/− mice. The experiments showed that mitochondrial mophology-, redox- and ATP-related gene expressions markedly differed between AAA and normal aortic tissues. AngII infusion induced gene expression in aortas from male wild-type and smcKlf5-/- mice was measured at 28 days after exposure to 1000 mg/kg/d AngII. Three independent experiments were performed for each experiment.