Optimal Numbers of F1 and F2 Animals Required to Screen 5,000 Mutagenized F1 Animals for Different Screen Parameters.

All values are for fully penetrant recessive mutations for which p = 0.75. Screen types are defined in the text. N/A, not applicable. All screens are for recessive mutants unless otherwise noted.aF1 animals are either individually plated at 1 F1 per plate, or plated in bulk (e.g. from a liquid culture) on one or a small number of plates.bα = 1.01, the amount of work to pick 1 F2 animal to a plate is, by definition, equal to 1; it is estimated that to score a visible mutant requires about 1/100 the amount of work of picking 1 F2 animal to a plate (α = 0.01), thus, α is the sum of these.cγ = 1 is, by definition, the work to pick one F1 animal to a plate using a worm pick.dγ = 0.001, it is estimated that the amount of work to bulk plate 1 F1 animal is equal to 1/1000 the amount of work to pick one F1 animal using a worm pick.eIn this case the values of α and γ essentially approach 0.