Data for: The curcuminoid, EF-24, reduces cisplatin-mediated reactive oxygen species in zebrafish inner ear auditory and vestibular tissues

Cisplatin is a widely used chemotherapy drug that can damage auditory and vestibular tissue and cause hearing and balance loss through the intracellular release of reactive oxygen species (ROS). Curcumin has anticancer efficacy and can also counteract cisplatin’s damaging effect against sensory tissue by scavenging intracellular ROS, but curcumin’s applicability is limited due to its low bioavailability. EF-24 is a synthetic curcumin analog that is more bioavailable than curcumin and can target cancer, but its effects against cisplatin-mediated ROS in auditory and vestibular tissue is currently unknown. In this study, we employed a novel zebrafish inner ear tissue culture system to determine if EF-24 counteracted cisplatin-mediated ROS release in two sensory endorgans, the saccule and the utricle. The zebrafish saccule is associated with auditory function and the utricle with vestibular function. Trimmed endorgans were placed in tissue culture media with a fluorescent reactive oxygen species indicator dye, and intracellular ROS release was measured using a spectrophotometer. We found that cisplatin treatment significantly increased ROS release compared to controls, but that EF-24 treatment did not alter or even decreased ROS release. Importantly, when equimolar cisplatin and EF-24 treatments are combined, ROS release did not increase compared to controls. This suggests that EF-24 may be able to prevent intracellular ROS release caused by cisplatin treatment in inner ear tissue.

顺铂是一种广泛使用的化疗药物，能够通过细胞内释放活性氧（ROS）损害听觉和前庭组织，从而导致听力及平衡功能的丧失。姜黄素具有抗癌活性，且能够通过清除细胞内的ROS来对抗顺铂对感觉组织的损害作用，但其应用受到其低生物利用度的限制。EF-24是一种比姜黄素具有更高生物利用度的合成姜黄素类似物，能够靶向癌症，但其对顺铂介导的ROS在听觉和前庭组织中的影响目前尚不清楚。在本研究中，我们采用了一种新型的斑马鱼内耳组织培养系统，以确定EF-24是否能够对抗顺铂介导的两个感觉终末器官——囊斑和椭圆囊中的ROS释放。斑马鱼囊斑与听觉功能相关，而椭圆囊与前庭功能相关。经过修剪的终末器官被放置在含有荧光活性氧指示染料的组织培养基中，通过分光光度计测量细胞内ROS的释放。我们发现，与对照相比，顺铂处理显著增加了ROS的释放，但EF-24处理并未改变甚至降低了ROS的释放。重要的是，当等摩尔量的顺铂和EF-24处理同时进行时，与对照相比，ROS的释放并未增加。这表明EF-24可能能够防止内耳组织中由顺铂治疗引起的细胞内ROS释放。