Data for: Study of the non-clinical healing activities of the extract and gel of Portulaca pilosa L. in skin wounds in wistar rats

Introduction: Portulaca pilosa L., belonging to the family Portulacaceae, is a common herbaceous plant in the Americas and in the Amazon, is popularly known as love-grown and is traditionally used as an aid in the treatment of burns, buds, insect bites and wound healing. This study aims to evaluate the non-clinical topical healing activity of the P. pilosa gel (GPP) and the propyleneglycol extract of P. pilosa (EPP) in Wistar rats.
Methods: For the healing activity, wistar rats were divided into the following groups: negative control (GVE - vehicle, 150 mg/kg), positive control (Fibrinase®- FIB, 100 U/kg), Portulaca pilosa gel 10%, (GPP, 150 mg/kg), and propylenglycollic extract of Portulaca pilosa (EPP, 150 mg/kg), which were submitted to a surgical procedure to obtain the wounds, and were treated topically for 7 days. After treatment, the treated area was removed and a histopathological analysis was performed.
Results: The EPP when analyzed in HPLC was able to identify the presence of gallic acid. EPP significantly modulated the tissue inflammatory response, presenting low number of inflammatory cells in the histopathological study. Treatment with EPP and GPP significantly stimulated angiogenesis and this response was superior to the fibrinase® group. Treatment with EPP and GPP significantly stimulated the proliferation of fibroblasts. The groups treated with EPP and GPP presented an organization pattern of the epidermis and dermis better than the control group, with a mild inflammatory process, with fibroblast proliferation and increased formation of collagen fibers.
Conclusion: Thus, from the results obtained it can be suggested that the phytochemical marker of the P. pilosa species for healing activity is gallic acid and, together with the macroscopic and microscopic findings triggered by the topical applications of EPP and GPP, it can be concluded that this plant species has topical healing activity, with great potential for use, since this pharmacological action is associated with a possible topical anti-inflammatory activity.

引言：Portulaca pilosa L.（毛叶酸模，拉丁学名），隶属于酸模科，为美洲及亚马逊地区常见的草本植物，俗称爱育草，传统上被用于烧伤、疖肿、虫咬及伤口愈合的辅助治疗。本研究旨在评估毛叶酸模凝胶（GPP）及毛叶酸模丙二醇提取物（EPP）在Wistar大鼠身上的非临床局部愈合活性。方法：为测试愈合活性，将Wistar大鼠分为以下几组：阴性对照组（GVE - 载体，150 mg/kg）、阳性对照组（Fibrinase®- FIB，100 U/kg）、毛叶酸模凝胶10%（GPP，150 mg/kg）和毛叶酸模丙二醇提取物（EPP，150 mg/kg）。各组大鼠均接受外科手术以获取伤口，并进行为期7天的局部治疗。治疗后，去除治疗区域并执行组织病理学分析。结果：EPP在高效液相色谱分析中能够识别出儿茶酸的存在。EPP显著调节了组织炎症反应，在组织病理学研究中显示出较低的炎症细胞数量。EPP和GPP治疗显著刺激了血管生成，且这种反应优于纤维蛋白酶组。EPP和GPP治疗显著刺激了成纤维细胞的增殖。EPP和GPP治疗组表皮和真皮的组织结构优于对照组，伴有轻微的炎症过程，成纤维细胞增殖和胶原纤维形成增加。结论：因此，根据所获得的结果，可以推测毛叶酸模物种的愈合活性植物化学标记为儿茶酸，并且结合EPP和GPP局部应用引起的宏观和微观发现，可以得出结论：该植物物种具有局部愈合活性，具有巨大的应用潜力，因为这种药理作用与可能的局部抗炎活性相关。