Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells

Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modelling cDC development is both crucial to study cDCs and harness their potential in immunotherapy. Here we present a novel in vitro cDC differentiation protocol using cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) co-cultured with bone marrow-derived murine mesenchymal cell line (MS5) engineered to co-express human FLT3L, SCF and CXCL12 (MS5_FS12) or MS5 engineered to express human GM-CSF (MS5_GM). In vitro generated CD1c+CD14+, CD14+CD16+, CD1c-CD14+, Clec9A+CD141+ and CD1c+CD206+ cells were collected at day 14. All cell types were analyzed in triplicates with up to 100 cells per sample.