OP-Strand-seq NA12878 libraries

Single cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one pot (OP) Strand-seq protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10-25 percent of the genome from a single cell with reduced costs and increased throughput. Here we provide high-coverage NA12878 libraries produced with OP-Strand-seq.