Transcriptomic analysis of mutant yeast strains deficient in Xrn1 nuclear import

The goal of the project was to study the transcription rates and mRNA levels, genome-wide, in several mutants in Xrn1 defetive in nuclear import. We used Genomic Run-On (GRO) experiment in wild type and xrn1 mutant strains.本项目的研究目标为：在若干核输入缺陷型Xrn1突变体中，开展全基因组范围内的转录速率与mRNA水平分析。本研究针对野生型菌株与xrn1突变菌株，采用基因组延伸运行（Genomic Run-On，GRO）实验技术。