Ebf2 Knockout Brown Adipose Tissue [RNA-Seq]

The helix-loop-helix transcription factor Early B-Cell Factor 2 (EBF2) is required for the differentiation and function of brown and beige adipocytes. The presumptive BAT in Ebf2 knockout (KO) animals has a white-fat like morphology and molecular profile. We examined the transcriptome of WT and Ebf2 KO BAT by high-throughput sequencing. Two control replicates (wildtype mice) and two experimental replicates (Ebf2 KO mice) were analyzed螺旋-环-螺旋转录因子早期B细胞因子2（Early B-Cell Factor 2, EBF2）是棕色脂肪细胞与米色脂肪细胞分化及功能发挥所必需的。在Ebf2基因敲除（knockout, KO）动物体内，其推定的棕色脂肪组织（brown adipose tissue, BAT）呈现出白色脂肪样的形态与分子特征。本研究通过高通量测序分析了野生型（wildtype, WT）与Ebf2 KO小鼠的BAT转录组，共对2份对照重复样本（野生型小鼠）及2份实验重复样本（Ebf2 KO小鼠）进行了分析。