John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10503, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D Deconvolved stack corresponds to raw data stack: CIL# 10502.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

一层人类皮肤成纤维细胞被感染了表达EGFP标记的TgCentrin2和mCherry标记的TgIMC1（内质膜复合支架蛋白）的弓形虫（Toxoplasma gondii）。使用Olympus IX70倒置显微镜结合API DeltaVision系统采集图像。实验目的：采用Olympus 100倍油镜，数值孔径为1.35。滤波器：EGFP，激发波长450-490nm，发射波长500-540nm；mCherry，激发波长555-592nm，发射波长600-665nm。双色反射镜：Chroma 89021。图像采集设备为CoolSnapHQ/ICX285。三维去卷积堆栈对应于原始数据堆栈：CIL# 10502。此图像为未发表图像，与PMID: 16518471中发表的图像相似。有关其他实验细节，请参阅相关出版物。