Bulk RNA sequencing data of macrophages treated with or without sFRP1

Total RNA of BMDMs was isolated using the Trizol reagent. RNA extracted from non-treated BMDMs was marked as control while RNA from sFRP1-treated BMDMs was marked as experiment group. Then, transcriptome sequencing and analysis were performed at Oebiotech Co. Ltd (Shanghai, China). Differential expression analysis was performed using DESeq2 (1.24.0) analysis. P value<0.05 and fold change>2 were set as the threshold for significantly differential expression. All the differentially expressed genes were named as "all DEGs" in the Mendeley repository and the significantly differential genes were named as "Significant DEGs".采用Trizol试剂提取骨髓源性巨噬细胞（Bone Marrow Derived Macrophages, BMDMs）的总RNA。将未经处理的骨髓源性巨噬细胞提取的RNA设为对照组，经分泌型卷曲相关蛋白1（Secreted Frizzled-Related Protein 1, sFRP1）处理的骨髓源性巨噬细胞提取的RNA设为实验组。随后，转录组测序与分析工作在中国上海的欧易生物（Oebiotech Co. Ltd）完成。采用DESeq2（版本1.24.0）进行差异表达分析，以P值小于0.05且差异倍数大于2作为显著差异表达的筛选阈值。所有差异表达基因在Mendeley存储库中被命名为"all DEGs"，显著差异表达基因则被命名为"Significant DEGs"。