Chromatin spreading in 3D through Insulator looping regulates genes through to H3K27me3 micro-domains

We used the technology of ChIP-sequencing to analyze changes in H3K27me3 levels in Drosophila Schneider S2 depleted of endogenous Beaf32 and expressing insulator mutants ('mut-Beaf32' or 'muBeaf') as compared to control cells expressing wild-type Beaf32 (Control; 'CTL') Overall design: Drosophila Schneider S2 cells either containing endogenous Beaf32 or either transfected by mutated insulator looping mutants (mut-Beaf32 ) have been depleted of endogenous Beaf32. Quantification of H3K27me3 has been done using ChIP-seq on both depleted and wild-type condition.