Single-cell RNA sequencing of bone marrow aspirate samples from multiple myeloma and its precursor conditions

To determine the cell types and their transcriptional alterations during multiple myeloma progression from its precursor conditions, ie. monoclonal gammopathy of undetermined significance (MGUS), and smoldering multiple myeloma (SMM), we used single-cell RNA sequencing (scRNA-seq) to analyze the bone marrow aspirate samples from 4 newly diagnosed multiple myeloma, 6 MGUS and 4 SMM patients as well as 5 healthy donors. Overall design: Sequential bone marrow aspirate samples were collected from 7 patients at different disease states and processed for scRNA-seq analysis. Bone marrow aspirate samples from 5 healthy donors were used as controls.