ChIP-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

To finely dissect the dynamic changes in the epigenome and chromatin state during T cell activation, we utilized the Jurkat cell line as a model and performed ChIP-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells, targeting various histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), RNA PolII, and CTCF. We performed ChIP-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells for distinct histone modifications and TFs. Anti-CD28 and anti-CD3 antibodies were utilized to activate T cells as previously described (Simeonov et al. Nature, 2017). Briefly, the culture plate was coated with 10 µg/ml of anti-CD28 (TONBO Biosciences, 40-0289) for 12 hours at 4°C prior to cell inoculation. Jurkat or CD4+ T cells were then seeded with 10 µg/ml of anti-CD3 (40-0038). The cells were harvested 24 hours later for subsequent experimental analysis. **Please note that the records have been updated as below on Sep 22, 2025: [1] both raw and processed data for GSM8522707-GSM8522720 have been replaced [2] additional sample records have been included